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D-Luciferin Potassium Salt
AOB2559
CAS: 115144-35-9
Chemical Name: (4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid pottasium salt
996 Items
Quantity Discount Table - Order More To Get More Price Discount
| Quantity | gram | Unit Price ($/mg or $/Unit) | Final Price |
|---|---|---|---|
| 1 | 5 | $148.75 | Total: $743.75 |
| 1 | 10 | $126.00 | Total: $1,260.00 |
| 1 | 25 | $106.75 | Total: $2,668.75 |
| 1 | 50 | $91.00 | Total: $4,550.00 |
| 1 | 100 | $78.75 | Total: $7,875.00 |
Overview
D-Luciferin Potassium Salt is the water-soluble, potassium salt form of D-luciferin—a bioluminescent substrate used widely in scientific imaging. Its role as a sensitive indicator of biological processes—from gene expression to disease tracking—makes it a foundational tool in areas like oncology, genetics, regenerative medicine, infectious disease, and drug discovery.
How It Works – The Biochemical Mechanism
Bioluminescent Reaction: D-luciferin undergoes ATP-dependent oxidative decarboxylation catalyzed by firefly luciferase, emitting visible light (around 560 nm).
- Light Output Proportionality: The light intensity correlates with luciferase concentration when luciferin is in excess—making it a reliable reporter for gene expression and other cell activities.
Primary Applications
1. In Vivo Bioluminescence Imaging (BLI)
Researchers use D-Luciferin to non-invasively image luciferase-expressing cells or animals (e.g., tumor growth, stem cell tracking, infection models) using CCD cameras.
2. Reporter Gene Assays
Luciferase genes (driven by promoters of interest) produce luminescence upon D-luciferin addition. This enables real-time studies of gene expression, circadian rhythms, toxicology, and more.
3. High-Throughput Screening (HTS)
In multiwell plate assays, D-Luciferin serves as a substrate to detect gene or protein activity via luminescence, facilitating large-scale compound screening.
4. Stem Cell & Regenerative Medicine Research
When stem cells are engineered to express luciferase, D-luciferin enables longitudinal tracking of their survival, migration, and differentiation in vivo.
5. Pathogen Tracking
Pathogens tagged with luciferase can be visualized in living hosts by administering D-Luciferin, useful for studying infection progression and treatment efficacy.
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6. ATP Quantitation and Metabolic Assays
The reaction’s requirement for ATP allows its use in ultrasensitive assays measuring ATP levels or metabolic activity.
Advantages of the Potassium Salt Form
Water Solubility: Readily dissolves in aqueous buffers and organic solvents, facilitating preparation for in vitro and in vivo use.
Low Background, High Sensitivity: Produces a high signal-to-noise ratio, making it ideal for detecting weak signals.
Validated and Reproducible: Proven over decades across multiple research areas, supported by numerous published protocols.
Stable with Proper Storage: Typically stored at –20 °C, protected from light and moisture. Solution stability is limited (e.g., <1 day when dissolved in PBS).
Chemical Properties
| Molecular Formula | C11H7KN2O3S2 |
| Molecular Weight | 318.41 |
| CAS Numbers | 115144-35-9 |
| Solubility | DMF: 16.7 mg/ml DMSO: 10 mg/ml Ethanol: 0.25 mg/ml PBS (pH 7.2): 10 mg/ml |
| Purity | 99% (HPLC) |
| IUPAC/Chemical Name | (4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid pottasium salt |
| InChl Key | UMBKGTQQGYPQBE-OGFXRTJISA-M |
| InChl Code | InChI=1S/C11H8N2O3S2.K/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;/h1-3,7,14H,4H2,(H,15,16);/q;+1/p-1/t7-;/m1./s1 |
| SMILES Code | O=C([O-])[C@H]1CSC(C(S2)=NC3=C2C=C(O)C=C3)=N1.[K+] |
Storage and Handling
0°C (short term), -20°C (long term), desiccated
References
1) Lembert, N. Firefly luciferase can use L-luciferin to produce light. Biochem. J. 317(1), 273-277 (1996).