Human Primary Mammary Fibroblasts Cells

Cell Details

Breast cancer is one of the malignant tumors that seriously affect women's health and even life-threatening. Its development is not only the role of tumor cells themselves, but also closely related to the tumor microenvironment. Breast cancer, one of the most common malignant tumors in women, is a malignant tumor that occurs in the mammary epithelial tissue. The onset is often associated with heredity. Breast cancer cells are highly heterogeneous and vary in cell morphology and size. 

Cell Characteristics

1) Cells are derived from human breast cancer tissue. 
2) Cell identification: ER immunofluorescence staining is positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: Irregular cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.