HRP conjugated Rabbit Anti-human β-Actin (ACTB) Monoclonal Antibody (clone 4C4)

HRP conjugated Rabbit Anti-human β-Actin (ACTB) Monoclonal Antibody (clone 4C4)

AP36775MC4H

500 Items

$154.00

$154.00 per 50 µl

Overview

DESCRIPTION

Species Reactivity

β-Actin from Human, Mouse, Rat, Pig

Immunogen

Full length recombinant human β-Actin protein

Purity

> 95% determined by SDS-PAGE

Formulation

0.05 M carbonate-bicarbonate buffer, 50% glycerol, pH 9.4

Size

50µl (concentration 1mg/mL)

APPLICATION

ELISA

Tested

WB

1: 1,000

IHC

N/A

Research Use or Further Manufacturing Only.

PREPARATION AND STORAGE

Preparation 

Monoclonal antibody is produced by immunizing rabbit with full length human β-Actin and purified using protein A resin.

Storage

This antibody is shipped at 4 oC. This product is stable for 12 months from date of receipt when stored at -20oC to -70oC. Avoid freeze/thaw cycles

BACKGROUND

β-actin is a member of highly conserved actins that are involved in cell motility, structure, and integrity. β-actin exists in two forms in cytoplasm: globular actin monomer (G-actin) with molecular weight approximately 42 kDa and fibrous polymer (F-actin). Due to its ubiquitous expression in eukaryotic cells, β-actin is widely used as a loading control for western blotting, markers for the integrity of cells. Defects in β-actin are a cause of dystonia juvenile-onset (DYTJ), often leading to abnormal postures, dopa-unresponsive dystonia, developmental malformations, and hearing loss. Research studies have also demonstrated that actin is associated with malignancies such as breast cancer and lymphoma.