Categories
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Overview
DESCRIPTION | |
Species Reactivity | Human, mouse, rat. Other species not tested. |
Immunogen | 216 aa recombinant protein of human HMGB1. |
Purification | Purified by peptide affinity column. |
Formulation | Lyophilized with 2% BSA and 0.02% NaN3. |
Size | 100ug/200µl |
APPLICATION | |
ELISA | 1:10,000 – 1:40,000 |
Western Blot | 1:200-1:1000 |
Immunohistochemistry | 1:50-1:500 |
For research use only. Optimal dilutions/concentrations should be determined by each laboratory for each application. | |
PREPARATION AND STORAGE | |
Reconstitution | Reconstitute in 200µl of sterile H2O. |
Storage | Shipped at ambient temperature. Store immediately at -20°C upon receipt. Avoid repeated freeze-thaw cycles. |
REFERENCES |
El Gazzar,M., Yoza,B.K., Chen,X., et al. Chromatin-specific remodeling by HMGB1 and linker histone H1 silences proinflammatory genes during endotoxin tolerance. Mol. Cell. Biol. 29 (7), 1959-1971 (2009). |