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Cell Culture
- Protein Control Ligand
- Pathway Inhibitors
- Enzyme Inhibitors
- Kinase Inhibitors
- Protease
- Synthase
- p18
- p38
- p53
- p70
- p90
- Peptidase
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- Ceramide Turnover Enzymes
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- Cyclic Nucleotide Turnover Enzymes
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- Hydroxylases
- Ubiquitin-Activating Enzyme
- Adenosine Deaminase
- Clathrin
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- Imidazoline receptor
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- Biomarkers and Buffer Solutions
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- Proteins and Antibodies
- Primary Cells
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- Natural Products
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Primary Liver Parenchyma Cell Culture System
PriMed-008
Molarity Calculation Cart®
HOW TO ORDER
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Product Description
The primary hepatocyte cell culture system is an ideal complete medium protocol designed for liver parenchyma cells of human, rat, mouse, rabbit and other mammals grown in vitro. This system is a bicarbonate-based buffer system that maintains an acid-base buffer system around pH 7.2 in a 5% CO2 atmosphere. The system is a sterile liquid mixing system containing essential and non-essential amino acids, vitamins, hormones, growth factors, trace elements and some other organic and inorganic compounds and a low concentration of fetal calf serum (5%). The culture system is a liquid culture system based on a fixed formula, which can provide a suitable balanced nutrient environment for liver parenchyma cells of human, rat, mouse, rabbit and the like cultured in vitro, and can selectively maintain the liver. The survival of parenchymal cells.
System Compostion
| NAME | VOLUME | CONCENTRATION | STORAGE |
| Primary hepatocyte basal medium | 500mL | 1x | 4°C, protected from light |
| Primary hepatocyte culture additive | 5mL | 100x | -20°C, protected from light |
| Fetal bovine serum (FBS) | 25mL | Final concentration 5% | -20°C, protected from light |
| Double antibody (penicillin/streptomycin, P/S) | 5mL | 100x | -20°C, protected from light |
Transportation and Preservation
Place in a cold box containing biological ice packs for low temperature transport (please store the components in the system according to the conditions in the above list)
Product Use
1) This product can only be used for scientific research
2) This product has not passed the audit for direct use of live animals and people.
3) This product has not passed the audit for in vivo diagnosis