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Cell Culture
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Primary Ovarian Granulosa Cell Culture System
PriMed-028
Molarity Calculation Cart®
HOW TO ORDER
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Product Description
The primary ovarian granulosa cell culture system is an ideal complete medium protocol designed for mammalian ovarian granulosa cells grown in vitro, such as human, rat, mouse, and rabbit. This system is a bicarbonate-based buffer system that maintains an acid-base buffer system around pH 7.2 in a 5% CO2 atmosphere. The system is a sterile liquid mixing system containing essential and non-essential amino acids, vitamins, hormones, growth factors, trace elements and some other organic and inorganic compounds as well as fetal bovine serum (10%). The culture system is a liquid culture system based on a fixed formula, which can provide a suitable balanced nutrient environment for ovarian granulosa cells of human, rat, mouse, rabbit and other mammals cultured in vitro, and can selectively promote ovarian particles. Amplification of cells.
System Compostion
| NAME | VOLUME | CONCENTRATION | STORAGE |
| Primary ovarian granulosa cell basal medium | 500mL | 1x | 4°C, protected from light |
| Primary ovarian granulosa cell culture additive | 5mL | 100x | -20°C, protected from light |
| Fetal bovine serum (FBS) | 50mL | Final concentration 10% | -20°C, protected from light |
| Double antibody (penicillin/streptomycin, P/S) | 5mL | 100x | -20°C, protected from light |
Transportation and Preservation
Place in a cold box containing biological ice packs for low temperature transport (please store the components in the system according to the conditions in the above list)
Product Use
1) This product can only be used for scientific research
2) This product has not passed the audit for direct use of live animals and people.
3) This product has not passed the audit for in vivo diagnosis