Human Primary Gingival Fibroblasts Cells

Cell Details

The retinal pigment epithelium is located between the choroid and the outer cell of the photoreceptor and is the transport channel for ions, water, nutrients and metabolic end products between the subretinal and choroidal vessels. The retinal pigment epithelium is involved in the retinol cycle, phagocytic detachment of photoreceptor extracellular nodes to maintain photoreceptor cell excitability, and secrete a variety of growth factors to help maintain the structural integrity of choroidal endothelial cells and photoreceptor cells.

Cell Characteristics

1) Tissue is derived from normal human eye tissue.

2) Cell identification: Cytokeratin-8 (CK-8) immunofluorescence staining was positive.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: epithelial-like, irregular cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.