Human Primary Choroidal Microvascular Endothelial Cells

Cell Details

The production of retinal blood vessels is formed by the balance of various positive and negative regulators produced by endothelial cells, outer membrane cells and astrocytes. Most blinding diseases such as diabetic retinopathy, retina in premature infants, etc. are associated with angiogenesis. The main physiological changes in human retinal microvascular endothelial cell lesions are retinal inflammation, retinal arteriosclerosis, and retinal hemorrhage.

Cell Characteristics

1) Source: Fresh eyeballs

2) Content: >1x10 6 /mL

3) Identification: Factor VIII (Von Willebrand factor) and CD31-specific antibody immunofluorescence

4) Purity: >90%

5) Pollution: negative for mycoplasma, bacteria, yeast and fungi

6) Specifications: 1mL frozen tube packagings

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.