Human Primary Corneal Endothelial Cells

Cell Details

The placenta is an organ that exchanges substances between mother and child that are formed by the combination of the embryonic membrane and the maternal endometrium during pregnancy. The fetus develops in the uterus and relies on the placenta to obtain nutrients from the mother, while the two sides maintain considerable independence. 

The placenta plays a vital role in the normal development of the fetus, and the formation of a good placental vascular network is a prerequisite for maintaining a normal pregnancy. As one of the main components of the placental vascular bed, placental microvascular endothelial cells participate in placental angiogenesis and remodeling, regulate vasomotor, and play an important role in maintaining blood flow homeostasis in the placental vascular bed. 

Cell Characteristics

1) Cells are derived from human placental tissue. 
2) Cell identification: Platelet-endothelial cell adhesion molecule (PECAM-1/CD31) or von Willebrand factor (vWF) was positive for immunofluorescence staining. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.