Human Primary Retinal Pigment Epithelial Cells

Cell Details

The chorion are the fetal part of the placenta, accounting for the majority of the placenta. The villus trophoblast of the full-term placenta of pregnancy consists mainly of syncytiotrophoblasts, the cell trophoblasts are only scattered, and the inner layer of the trophoblast is the basement membrane. 

Trophoblast cell proliferation and dysregulation of function are closely related to the occurrence and development of various pregnancy-related diseases; chorionic trophoblast cells are the main tissue structure that constitutes the placental barrier, while placenta is a substance exchange and metabolism during pregnancy, secreting hormones and defense Important organs such as functions.

Cell Characteristics

1) Cells are derived from human normal placental tissue. 
2) Cell identification: Immunofluorescence staining of broad-spectrum keratin (PCK) or cytokeratin 7 (CK-7) was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.