Human Primary Amniotic Epithelial Cells

Cell Details

The umbilical cord is the tubular structure of the mammal that connects the fetus to the placenta. The umbilical cord that passes through the urinary tract is the umbilical artery and the umbilical vein, and the blood vessels of the yolk sac are the umbilical mesenteric artery and the umbilical mesenteric vein. In the uterus, the capillaries of the uterine artery in the mother part of the placenta are close to the fetal capillaries of the part of the placenta, where CO2 and O2 are carried out between the mother and the fetus, and the metabolites are metabolic wastes and nutrients. exchange. The umbilical artery transports the waste from the fetus to the placenta, which transports O2 and nutrients from the placenta to the fetus. 

Vascular smooth muscle is the cellular basis for many major vascular diseases; abnormally increased growth potential of vascular smooth muscle cells plays a decisive role in the development of vascular disease. Since the umbilical cord is a waste during childbirth, and the method of isolating human umbilical cord smooth muscle cells from the umbilical cord is relatively mature, the umbilical arterial smooth muscle cells cultured in vitro are used as model cells for studying blood vessels.

Cell Characteristics

1) Cells are derived from human umbilical cord tissue. 
2) Cell identification: Smooth muscle actin (α-SMA) immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: spindle cells, irregular cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.