Human Primary Type II Alveolar Epithelial Cells

Cell Details

Type II alveolar cells (ATII), also known as granule alveolar cells, are scattered between ATI alveolar cells (ATI) and their adjacent alveolar septal junctions. Its small size, cuboidal, slightly protruding into the alveolar space. The nucleus is large and round, the cytoplasm is stained lightly, and the vacuoles are common in the cytoplasm. More than ATI, ATII accounts for 14% to 16% of the total number of alveolar epithelial cells, but only covers 5c/o of alveolar surface. ATII is much smaller than ATI, with a population of about 900um3. There are more short fluff on the free surface of the cell, especially at the edge of the cell. The cell surface has MPA lectin, which specifically reacts with α-galactose residues. Adjacent cells are connected by tight junctions or intermediate connections, with more mitochondria and rough endoplasmic reticulum in the cytoplasm, as well as multivesicular, lysosome and lamellar [1]. ATII is a "stem cell" of alveolar epithelial cells, which is versatile: it can proliferate into new ATII, and can also differentiate into other upper cells such as ATI; the function of synthesizing and secreting surfactants; pulmonary water transport function; powerful immune function. These functions are inextricably linked to the following diseases.

Cell Characteristics

1) Cells are derived from human normal lung tissue. 
2) Cells were identified: Pulmonary surfactant protein A (SP-A) or a pulmonary surfactant protein C (SP-C) immunofluorescence light stained positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8ml cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research

2) This product has not passed the audit for direct use of live animals and people.

3) This product has not passed the audit for in vivo diagnosis