Human Primary Pulmonary Artery Endothelial Cells

Cell Details

Pulmonary artery vascular endothelial cells are a multifunctional cell, especially for the non-respiratory function of the lungs. When it is damaged, pulmonary vascular permeability leads to pulmonary edema, which is of great significance in the mechanism of adult respiratory distress syndrome. However, in the complex environment in which multiple factors interact together, it is difficult to conduct an in-depth study on the function and metabolism of pulmonary vascular endothelial cells and the pathogenesis of lesions. The in vitro culture system of primary pulmonary artery endothelial cells helps to study the function of pulmonary vascular endothelial cells under specific in vitro conditions.

Cell Characteristics

1) Tissue is derived from normal human pulmonary tissue.

2) Cell identification: von von Willebrand factor (vWF) was positive for immunofluorescence staining.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research

2) This product has not passed the audit for direct use of live animals and people.

3) This product has not passed the audit for in vivo diagnosis