Human Primary Pulmonary Artery Smooth Muscle Cells

Cell Details

The pulmonary artery originates from the right ventricle, obliquely to the left upper rear before the aorta, and divides into left and right pulmonary arteries under the aortic arch, and enters the lung through the hilar. Since the pulmonary artery is connected to the right ventricle that delivers venous blood, the pulmonary artery is an artery, but it delivers venous blood. Vascular smooth muscle cells are connected to each other to form a tubular structure; functionally, continuous contraction can be produced to maintain the original shape against the applied load. The cells expressed on the surface of the calcium channel expressed ICAM-1 and VCAM-1, which are involved in the vascular wall inflammatory response. The pulmonary aortic smooth muscle cells cultured in vitro are fusiform, star-shaped or irregular.

Cell Characteristics

1) Tissue is derived from normal human pulmonary tissue.

2) Cell identification: Smooth muscle actin (α-SMA) immunofluorescence staining was positive.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: long spindle cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research

2) This product has not passed the audit for direct use of live animals and people.

3) This product has not passed the audit for in vivo diagnosis