Human Primary Aortic Valve Mesenchymal Cells

Cell Details

The aortic valve is placed between the left ventricle and the aorta, and the blood injected into the aorta is prevented from flowing back into the left ventricle. Aortic valve calcification can cause aortic stenosis and insufficiency, and it is an important pathological change in elderly patients with degenerative valvular heart disease, and its incidence increases with age. Studies have shown that phenotypic transformation of stromal cells to urban solid cell-like cells may be one of the pathological changes of aortic valve calcification.

Cell Characteristics

1) Tissue is derived from normal aortic tissue of a human.

2) Cell identification: Positive staining of smooth muscle actin (α-SMA) or vimentin (Vimentin).  

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: long fusiform, irregular cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research

2) This product has not passed the audit for direct use of live animals and people.

3) This product has not passed the audit for in vivo diagnosis