Human Primary Esophageal Smooth Muscle Cells

Cell Details

The esophagus is the digestive tract between the pharynx and the stomach. The esophageal structure of the mammal is divided into four layers from the inside to the outside: the mucosa, the submucosa, the muscular layer, and the outer membrane. Esophageal smooth muscle is concentrated in the muscular layer, only in the lower 2/3 segment of the esophagus. One third of the skeletal muscle and smooth muscle are mixed in the middle segment, and the lower third segment is all smooth muscle, while the upper third is all skeletal muscle. In the movement of the esophagus, the upper striated muscle is dominated by the glossopharyngeal nerve and the vagus nerve. These motor neuron terminals enter the skeletal muscle in the form of motor endplates. The vagus nerve still dominates the smooth muscle of the rest of the esophagus. The pre-ganglionic fiber ends are synaptic with the ganglion cells of the inner lining of the esophageal wall, and then the posterior fibers innervate the smooth muscle cells. During swallowing, the swallowing center excites the fibers through the above-mentioned motor neurons and the vagus nerve, causing the muscles of various segments of the esophagus to creep. The inner plexus of the esophageal wall can control the esophageal sin without relying on external nerves move.

Cell Characteristics

1) Cells are derived from human normal esophageal tissue. 

2) Cell identification: Smooth muscle actin (α-SMA) immunofluorescence staining was positive. 

3) The purity of the identified cells is higher than 90%. 

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: long spindle cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.