Human Primary Small Intestinal Fibroblasts Cells

Cell Details

Small intestine is located in the abdomen, the upper end is connected to the pylorus and the stomach, and the lower end is connected to the large intestine through the cardia. It is the main place for food digestion and absorption. The small intestine is generally divided into three segments according to the shape and structural changes, namely the duodenum, jejunum and ileum. The structure of the small intestine wall is generally divided into 4 layers, from the outside to the inside: serosal layer, smooth muscle layer, submucosa and mucosa. The mucosal layer is further divided into three layers: close to the submucosa is a layer of smooth muscle called the mucosal muscle layer. Followed by connective tissue, also known as the lamina propria. Finally, facing the intestinal lumen is a layer of columnar epithelial cells composed of mucosa. 

Fibroblasts are the most common cells in connective tissue. Under electron microscope, abundant rough endoplasmic reticulum, free ribosome and Golgi complex are found in the cytoplasm of fibroblasts, indicating that they have the function of synthesizing and secreting proteins. One of the main functions of fibroblasts is the synthesis of collagen and other extracellular matrices, which play an important role in the process of tissue and organ fibrosis. 

Cell Characteristics

1) Cells are derived from surgically resected normal small intestine tissue. 
2) Cell identification: Fibronectin or vimentin was positive for immunofluorescence staining. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: fibroblast-like cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.