Human Primary Hepatic Stellate Cells

Cell Details

Hepatic stellate cells are located in the gap of the Disse, closely adjacent to the sinusoidal endothelial cells and hepatocytes. Its shape is irregular, the cell body is round or irregular, and often protrudes from several hepatic sinusoids around the hepatic sinus. 

Under normal circumstances, hepatic stellate cells are characterized by a static type rich in vitamin A lipid droplets. Their functions include: metabolism and storage of vitamin A, storage of fat, synthesis and secretion of collagen and glycoprotein, proteoglycan and other matrix components, synthetic matrix metal. Protease and its tissue inhibitors, expression of cytokines and receptors, and involvement in hepatic sinusoidal blood flow regulation. 

Hepatic stellate cells are the main source of extracellular matrix, and hepatic stellate cells are normally at rest. When the liver is damaged by inflammation or mechanical stimulation, the hepatic stellate cells are activated, and its phenotype changes from static to activated. Hepatic stellate cells activate and transform into myofibroblast-like cells, various fibrogenic factors. HSC was used as the final target cell. On the one hand, activated hepatic stellate cells participate in the formation of hepatic fibrosis and the reconstruction of intrahepatic structures by proliferating and secreting extracellular matrix, and on the other hand, the intrahepatic sinus pressure is increased by cell contraction. 

Cell Characteristics

1) The cells are derived from human normal liver tissue. 
2) Cell identification: CD95L immunofluorescence staining was positive (quiescent state); desmin (Desmin) or smooth muscle actin (α-SMA) immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: long fusiform, stellate cells, adherent culture. 

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.