Human Primary Sinusoidal Endothelial Cells

Cell Details

The sinusoidal endothelial cells are the most abundant cells in the liver non-parenchymal cells, accounting for about 70% of the total number of non-parenchymal cells in the liver. They are significantly different in phenotype and function from ordinary capillary endothelial cells. There is no intercellular connection between the sinusoidal endothelial cells, and there are few substances in the basement membrane under the cells. Therefore, the sinusoidal permeability is high, which is beneficial to regulate the substance exchange.

Unlike the self-replication of hepatocytes, the new LSECs in liver regeneration mainly come from the differentiation of other cellular components inside and outside the liver. Many studies have confirmed the bone marrow-derived substitution of LSECs during liver regeneration. Endothelial progenitor cells are the major cellular components involved in this process.

Aperture is the most characteristic structure of sinusoidal endothelial cells, from <10nm to 1~2μm range, since under physiological conditions in the presence and absence of endothelial fenestration complete base structure of the membrane, composed of a sinusoidal endothelial cells The wall of the hepatic sinus is the only capillaries in the capillary wall of the whole body that lack the basement membrane. Except for the blood cells in the sinus, the plasma components can enter the Disse gap from the window to exchange material.

Cell Characteristics

1) The cells are derived from human normal liver tissue.

2) Cell identification: von von Willebrand factor (vWF) was positive for immunofluorescence staining.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.