Human Primary Rectal Fibroblasts Cells

Cell Details

The rectum as a large intestine 15cm upward from the anal verge. The rectum is multi-fat, no longitudinal, and is located on the dorsal side of the bladder and reproductive organs. The cross-section of the rectum is from the inside to the outside: mucous membrane (epithelial layer, lamina propria, mucosal muscle layer), submucosa, muscular layer, adventitia. 

Fibroblasts are the main cellular components of loose connective tissue, and tumor stroma plays an important role in the development of tumors. Fibroblasts are important components of tumor stroma and are closely related to tumor recurrence and metastasis. The activation of fibroblasts in tumor stroma is a cancer-associated fibroblast. It is an important process in the process of tumor cell-fibroblast interaction, and it is the key to the role of fibroblasts in tumor regulation. At the same time, changes in fibroblasts are also closely related to many other rectal diseases.

Cell Characteristics

1) Cells are derived from normal rectal tissue that has been surgically removed. 

2) Cell identification: Fibronectin or vimentin was positive for immunofluorescence staining.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: fibroblast-like cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.