Human Primary Gallbladder Microvascular Endothelial Cells

Cell Details

The gallbladder is a pear-shaped capsular bag structure located behind the liver under the right rib, which has the function of enriching and storing bile. The gallbladder wall consists of three layers: the mucosa, the muscular layer and the outer membrane. Microvessels act as the most widely distributed blood vessels. It is a blood vessel that connects the arteries and venules. They branch and fit into each other. The pipe wall is thin and the permeability is strong. Its function is to facilitate the exchange of substances between blood and tissue. The microvascular wall is composed mainly of a layer of endothelial cells and a basement membrane. The fine capillary cross section is surrounded by an endothelial cell.

Cell Characteristics

1) Cells are derived from human normal gallbladder tissue. 
2) Cell identification: vWF immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.