Human Primary Gallbladder Arterial Endothelial Cells

Cell Details

The gallbladder is a pear-shaped capsular bag structure located behind the liver under the right rib, which has the function of enriching and storing bile. The gallbladder wall consists of three layers: the mucosa, the muscular layer and the outer membrane. The arteries are composed of the inner membrane, the media and the outer membrane, wherein the inner membrane is mainly composed of endothelial cells.

Cell Characteristics

1) Cells are derived from human normal gallbladder tissue. 
2) Cell identification: vWF immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: epithelial-like, polygonal cells, adherent culture

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.