Human Primary Renal Tubular Epithelial Cells

Cell Details

An elongated epithelial tubule connected to the renal tubule wall with reabsorption and excretion . The renal tubule is divided into three parts according to different morphological structures, distribution position and function; proximal tubule, medullary ridge and distal end small tube.

The average length of the renal tubules is about 30 to 50 mm , which are composed of a single layer of epithelium. Ischemia, infection and poison can cause degeneration and necrosis of renal tubular epithelial cells, leading to renal dysfunction. Aldosterone, vasopressin, atrial natriuretic peptide, parathyroid hormone, etc., can also lead to changes in renal tubular function. Because of the different structures and functions of the renal tubules in different segments, the performance varies with dysfunction.

Cell Characteristics

1) The cells are derived from human normal kidney tissue.

2) Cells were identified: a broad spectrum cytokeratin (PCK) or cytokeratin -18 (CK-18 is) stained positive by immunofluorescence.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.