Human Primary Glomerular Mesangial Cells

Cell Details

Each nephron consists of a renal corpuscle and a renal tubule. The renal corpuscle has a capillary mass called the glomerulus, which is formed by the branches of the renal artery. There is a small capsular around the glomerulus. The renal capsule is divided into two layers, and there is a cyst between the two layers communicating with the lumen of the renal tubule. The renal tubules merge into a collecting tube. Several collection tubes merge into a nipple tube, and urine flows into the renal sputum.The glomerulus is a blood filter, and the glomerular capillary wall constitutes a filter membrane.

The mesangium is composed of mesangial cells and mesangial matrix, which is the axial tissue between the lobules of the glomerular capillary plexus and directly adjacent to the endothelium of the capillaries, which plays a supporting role between the capillaries in the glomerulus. Mesangial cells have multiple functions, and there are contractile filaments in the cells, which regulate the glomerular capillary surface area by stimulating the contraction of the filaments, thereby controlling the glomerular blood flow. Mesangial cells maintain the adjacent basement membrane and act as a scaffold for the glomerular capillaries. Mesangial cells have the ability to phagocytose and remove foreign bodies, such as immune complexes, abnormal proteins and other particles.

Cell Characteristics

1) The cells are derived from human normal kidney tissue.

2) Cell identification: Desmin or Vimentin immunofluorescence staining was positive.

3) The purity of the identified cells is higher than 90% .

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: long fusiform, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.