Human Primary Kidney Foot Cells

Cell Details

The glomerulus is a blood filter, and the glomerular capillary wall constitutes a filter membrane. The glomerular filtration membrane has three layers from the inside to the outside: the inner layer is the endothelium, the middle layer is the glomerular basement membrane, and the outer layer is the epithelial cell layer. The epithelial cells are also called the podocytes, and the irregular protrusions are called the foot processes. In many narrow gaps, the blood is filtered through the filter and the filtrate is introduced into the renal capsule. Under normal circumstances, most of the protein in the blood can not be filtered and retained in the blood, only small molecules such as urea, glucose, electrolytes and some small molecules can be filtered.

The renal foot cells, glomerular epithelial cells, which are attached to the outside of the glomerular basement membrane, together with the glomerular basement membrane and the glomerular basement membrane, constitute the glomerular hemofiltration barrier. Since the kidney podocytes of the normal adult body are terminally differentiated cells, the primary cells cultured in vitro cannot proliferate.

The podocytes are star-shaped and multi-spike, and the cell bodies are large. Many protrusions protrude from the cell body, and the fingers overlap the outer surface of the glomerular basement membrane, and are connected to the glomerular basement membrane through adhesion molecules and proteoglycan molecules. Under normal conditions, podocytes can secrete type IV collagen and fibronectin, the main components of the glomerular basement membrane, and secrete matrix metalloproteinases that degrade the glomerular basement membrane under the stimulation of renal fibrosis. Cathepsins play an important role in the metabolic balance of the glomerular basement membrane.

Cell Characteristics

1) The cells are derived from human normal kidney tissue.

2) Cell identification: Immunofluorescence staining was positive for broad-spectrum keratin (PCK) or WT-1 (Wilm's TumorProtein).

3) The purity of the identified cells is higher than 90% .

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: Irregular, containing podocytes, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.