Human Primary Bladder Epithelial Cells

Cell Details

The bladder is a urine storage organ. It is a cystic structure composed of smooth muscles. It is located in the pelvis and has a rear end opening that communicates with the urethra. There is a sphincter at the junction of the bladder and the urethra, which can control the discharge of urine.

The bladder wall is divided into three layers: the serosal layer, the muscular layer, and the mucosal layer. The bladder epithelial cells are mainly from the mucosal layer, which is a very thin layer of transitional epithelial tissue, and the ureter and urethra mucosa are coherent. The mucosa is very smooth in the triangle because it is tightly connected to the underlying muscles, but in other areas it has significant wrinkles, and when the bladder is full, the wrinkles disappear. The mucosal layer has glandular tissue, especially in the neck and triangle of the bladder.

Since the urinary tract metastasis epithelium will fall off and be discharged together with the urine, it is relatively easy to obtain, and it is a seed cell for urinary tissue engineering.

Cell Characteristics

1) The cells are derived from human normal bladder tissue.

2) Cell identification: broad-spectrum keratin (PCK) immunofluorescence staining was positive.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.