Human Primary Prostatic Epithelial Cells

Cell Details

The prostate is a male-specific gonadal organ composed of glandular tissue and muscle tissue. In the middle of the prostate gland, there is a urethra passing through, and the upper urethra is kept. Therefore, the prostate is sick and urination is affected first. The prostate is a secretory gland with both internal and external secretory functions. As the exocrine gland, the prostate secretes prostatic fluid, which is the main component of semen; as the endocrine gland, the hormone secreted by the prostate is called "prostaglandin."

There is a layer of film on the surface of the prostate. It contains more elastic fibers and smooth muscles. These components can protrude into the gland and form the scaffold of the prostate. The parenchyma of the prostate consists of 30-50 complex vesicular glands. A total of 15-30 catheters are open to the urethra. On both sides, according to the distribution of glands, it can be divided into mucosal glands, submucosal glands and main glands. The acinar epithelium is a single-layered cubic, single-layer column or pseudo-stratified column; the morphology is different, and the glandular cavity is irregular; the coagulation body is usually coagulated, and the secretion of epithelial cells is concentrated.

Cell Characteristics

1) The cells are derived from human normal prostate tissue.

2) Cell identification: broad-spectrum keratin (PCK) immunofluorescence staining was positive.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.