Human Primary Thyroid Fibroblasts Cells

Cell Details

The thyroid gland is a very important gland of vertebrates and belongs to the endocrine organs. In mammals it is located below the thyroid cartilage of the neck, on both sides of the trachea. The human thyroid gland resembles a butterfly. The thyroid controls the speed of energy use, makes proteins, and regulates the body's sensitivity to other hormones.

The thyroid has two layers of membrane: the anterior fascia of the trachea surrounds the thyroid gland to form the thyroid sheath, which is called the thyroid pseudomembrane ; the outer membrane of the thyroid itself extends into the glandular parenchyma, and the gland is divided into several small leaves, namely the fibrous sac, also known as the thyroid gland. The capsule, which is the main gathering place for thyroid fibroblasts. There are loose connective tissue, blood vessels, nerves and parathyroid glands in the gap between the gland sheath and the fibrous capsule.

Cell Characteristics

1) The  cells are derived from human normal thyroid tissue.

2) Cells were identified: fibronectin (Fibronectin) or vimentin (of Vimentin) stained positive by immunofluorescence.

3) The purity of the identified cells is higher than 90% .

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

5) Cell growth mode: long spindle cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.