Human Primary Pancreatic Stellate Cells

Cell Details

The stellate cells in the normal pancreatic tissue are in a static state. The static pancreatic stellate cells contain vitamin A lipid droplets and oleic acid retinol, and have the function of ingesting and esterifying retinol, but do not have profibrotic effects.

When various pancreatic damage-causing stimulating factors activate pancreatic stellate cells, their intracellular lipid droplets disappear and transform into myofibroblast-like phenotypic cells, which are characterized by fibrogenic and smooth muscle cells: with extracellular matrix. The main components such as type I, type III collagen, fibronectin, and laminin antibodies are strongly positive staining reactions. It suggests a large amount of synthesis and deposition of extracellular matrix. This process has recently been confirmed in animal models and pancreatic tissue in patients with chronic pancreatitis. In situ hybridization showed that cells that were only positive for α-SMA staining in the fibrotic pancreas were the main source of collagen production.

Cell Characteristics

1) The cells are derived from human normal pancreatic tissue.

2) Cell identification: Desmin or smooth muscle actin (α- SMA) immunofluorescence staining was positive.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1 , HBV , HCV , mycoplasma, bacteria, yeast, and fungi.

5) Cell growth mode: long fusiform, stellate cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.