Human Primary Tonsilla Fibroblasts Cells

Cell Details

The fallopian tube is located in the pelvic cavity of the human body. There are two general people, and the left and right fallopian tubes are located on the side of the uterus. They extend outward from the lateral horn of the uterus, parallel to the uterus end of the ovary, then along the ovarian mesangial edge to the oviduct end of the ovary, and arched over the ovary, then down, inward, and terminate On the free edge of the ovary and the upper part of its inner side.

The epithelium of the mucosal layer is composed of a single layer of high columnar cells, which can be divided into four different types: ciliated cells, secretory cells, wedge cells, and undifferentiated cells.

The fallopian tube is an important place for egg transport, storage, and capacitation, as well as oocyte adoption, transport, maturation, fertilization, and early embryo development. In vitro culture of oviduct epithelial cells can be used for feeder cells and co-culture with embryos to overcome the developmental arrest of embryos. Therefore, in vitro culture of oviduct epithelial cells can not only further understand the factors affecting the changes of reproductive microenvironment, but also establish a system of co-culture of oviductal epithelial cells and embryos to study the effects of oviductal epithelial cells on the development of embryos in vitro.

Cell Characteristics

1) The cells are derived from human normal fallopian tube tissues.

2) Cell identification: Cytokeratin- 17 (CK-17) was positive for immunofluorescence staining.

3) The purity of the identified cells is higher than 90% .

4) Does not contain HIV-1 , HBV , HCV , mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: epithelioid, polygonal cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.