Human Primary Endometrial Epithelial Cells

Cell Details

The mammary gland is located between the shallow and deep layers of the subcutaneous superficial fascia. The superficial fascia extends into the mammary gland to form a cord-like leaflet interval, one end connected to the pectoral fascia and the other end attached to the skin, and the mammary gland is fixed in the subcutaneous tissue of the chest. The breast gland is composed of 15-20 glandular leaves, each gland is divided into several glandular lobules, each gland leaflet is composed of 10-100 acinar cells, which are closely arranged around the small milk duct, acinar The opening is connected to the small milk duct. 

Mammary epithelial cells are derived from the lobule of the breast. Together with glandular ducts and adipose tissue, they form complex network structures in the mammary gland. Mammary epithelial cells undergo a series of growth, migration and differentiation in the birth, development and pregnancy of humans and animals. Deregulation of hormone levels, changes in extracellular matrix, and other genetic factors can cause malignant growth of mammary epithelial cells, ultimately leading to breast cancer. Understanding the characteristics of breast epithelial cells can help us understand the case mechanisms of breast cancer and identify new targets for treatment.

Cell Characteristics

1) Cells are derived from human normal breast tissue. 
2) Cell identification: Cytokeratin-8 (CK-8) immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: epithelioid, polygonal cells, adherent culture.  

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.