Human Primary Breast Tumor Stem Cells

Cell Details

The spermatogonia is located on the basement membrane of the seminiferous tubule. It not only maintains its own number, but also differentiates into adult stem cells that produce spermatocytes. It is the only immortalized cell in the male body that can undergo mitosis and transfer genetic material to the next generation. Therefore, in vitro culture of spermatogonia provides a basis and premise for further research on the function of spermatogonial cells.

Cell Characteristics

1) Tissue is derived from normal testicular tissue of a human.

2) Cell identification: c-kit immunofluorescence staining was positive.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: round cells, irregular cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.