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1) Click the Molarity Calculation Cart® button.
2) Specify the Concentration and Volume of your desired Molarity.
3) Click Calculate.
4) Your desired Quantity will be calculated and will be put under Quantity: (only for mg)
5) Click Add to Cart and then Proceed to checkout to complete the order. You will receive the quantity as you ordered as solvent-free form.
Alert: If you want to make a solution with a solvent (such DMSO), please add instructions in the notes section (such as 10 nM/1 mL in DMSO) and we will pack and deliver the product as a solution (such as 10 nM solution in 1 mL DMSO). If the product cannot be dissolved in DMSO or you would like to specify your own preferred solvent, please add detailed instructions in the notes section. All solutions must be shipped with an ice bag, costing an additional fee of $20 for S&H.
HUM-f015
Each vial contains >5x105 cells in 1mL volume
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Cell Details
The vas deferens are a pair of elongated tubes, one on each side, each about 40 cm in length. One end of the vas deferens communicates with the epididymis tube , and the other end merges with the seminal vesicle tube to form an ejaculatory duct that opens into the posterior urethra. The vas deferens also store a portion of the mature sperm.
The vas deferens is a muscular duct with a thick wall and a narrow lumen. The wall of the tube consists of three layers: mucosa, muscular layer and outer membrane. Among them, the muscle layer is thicker, consisting of three layers of smooth muscles in the medial longitudinal, middle ring and lateral longitudinal , mainly composed of smooth muscle cells.
Cell Characteristics
1) Cells are derived from human normal vas deferens.
2) Cell identification: α-SMA immunofluorescence staining was positive.
3) The purity of the identified cells is higher than 90%.
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
5) Cell growth mode: long fusiform, irregular cells, adherent culture.
Transportation and Preservation
Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.
1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.
Product Use
1) This product can only be used for scientific research
2) This product has not passed the audit for living animals and humans directly.
3) This product has not passed the audit for in vivo diagnosis.