Human Primary Spermatogonia Cells

Cell Details

The epididymis is a most tortuous, small organ pipe configuration, is connected to one side of the vas deferens, the side connected to the testis of the seminiferous tubules. When the sperm leaves the testicles, it runs into the epididymis and continues to mature. In addition to storing sperm, the epididymis can also secrete epididymal fluid, which contains certain hormones, enzymes and specific nutrients that help sperm maturation. The epididymis tube consists of the mucosa, muscle layer and outer membrane. Among them, the mucosal layer is mainly composed of mucosal epithelial cells. 

Cell Characteristics

1) Tissues are derived from normal epididymis tissue of humans. 
2) Cell identification: PCK immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: paving stone, polygonal cells, adherent culture. 

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.