Human Primary Testicular Albuginea Fibroblasts Cells

Cell Details

Skin refers to the tissue on the surface of the body that is wrapped around the muscles. It is the largest organ in the body and is mainly responsible for protecting the body, perspiration, feeling hot and cold. The skin consists of the epidermis, dermis and subcutaneous tissue.

Fibroblasts belong to interstitial cells differentiated from mesoderm. Since these cells are very easy to culture, they have been widely used in cell and molecular biology research. In general, fibroblasts are capable of secreting extracellular matrices such as type I and type III collagen, and studies have shown significant differences in fibroblasts in different organs. When the wound is repaired, the dermal fibroblasts change from a proliferative, migratory phenotype to a contractile, remodeling the phenotype of the matrix, and at the same time, they secrete a large amount of hyaluronic acid to cope with the inflammatory response during repair.

Cell Characteristics

1) The cells are derived from normal foreskin tissue that has been surgically removed.

2) Cell identification: Fibronectin or vimentin was positive for immunofluorescence staining.

3) The purity of the identified cells is higher than 90%.

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.

5) Cell growth mode: fibroblast-like cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.