Human Primary Epididymal Epithelial Cells

Cell Details

Adipose tissue is mainly composed of a large number of clustered fat cells, which are separated into small leaves by thin layer of loose connective tissue. The reticular fibers in the adipose tissue are well developed.

Microvascular endothelial cell damage has been regarded as the pathological basis for the development of various diseases and syndromes such as trauma, infection, shock, tumor, and vascular disease. Once the microvascular endothelial cells are damaged, it will inevitably affect or even destroy the normal biological functions of microvascular endothelial cells, causing various diseases. There is a very close relationship between microvascular endothelial cell connections and vascular permeability. Microvascular endothelial cells synthesize and secrete prostacyclin, nitric oxide, endothelium-derived hyperpolarizing factor and endothelin to maintain the normal state of blood vessels.

Cell Characteristics

1) Cells are derived from human normal adipose tissue.

2) Cell identification: von von Willebrand factor (vWF) was positive for immunofluorescence staining. 

3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: epithelioid, polygonal cells, adherent culture. 

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.