Human Primary Epididymal Fibroblasts Cells

Cell Details

The dermal papilla cells are located at the base of the hair follicle and are a type of fibroblast. In the early stage of hair follicle development, the dermal cells send a first dermal signal to the monolayer epithelial cells, stimulating the local formation of the hairy substrate in the epithelium. Subsequently, the hair substrate cells emit a first epidermal signal to the lower dermis, which induces the formation of a cluster of aggregated cells composed of fibroblasts. During this process, the hair matrix cells gradually envelop the agglutinated cell mass to form mature dermal papilla cells. As an important cell group in hair follicles, the molecular mechanism and clinical application of dermal papilla cells are gradually being recognized and analyzed.

Cell Characteristics

1) Cells are derived from normal scalp tissue that has been surgically removed. 

2) Cell identification: Fibronectin was positive for immunofluorescence staining. 

3) The purity of the identified cells is higher than 90%. 

4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 

5) Cell growth mode: fibroblast-like cells, adherent culture.  

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.