Human Primary Keratinocytes Cells

Cell Details

The hair follicle is a saclike tissue surrounding the root of the hair. The inner layer is the epithelial tissue hair follicle, the outer layer is the connective tissue hair follicle, the inner layer is connected to the epidermis, and the outer layer is connected to the dermis. 

As an important skin accessory organ, the most prominent feature of hair follicles is that they are always in the cyclical cycle of growth, degeneration and rest periods. In the morphology and periodic circulation of hair follicles, keratinocytes of hair follicles act as a special type of keratinocytes, which are rapidly differentiated, proliferated or apoptotic, and then induced by some cytokines or signaling factors secreted by dermal papilla cells. The hair follicle enters the growth phase or the period of regression.

Cell Characteristics

1) Cells are derived from human scalp tissue. 
2) Cell identification: broad-spectrum keratin ((PCK) immunofluorescence staining is positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: epithelioid cells, adherent culture.   

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.