Human Primary Preadipocyte Cells

Cell Details

The ligaments which are dense connective tissues dominated by elastic fibers. Coarse elastic fibers or bundled in parallel, such as ligaments and ligamentum flavum. The white banded connective tissue of the ligament is tough and elastic, which can connect the bones together and fix the position of certain organs such as liver, spleen and kidney. 

The ligament is attached to the movable part of the bone, but its range of motion is limited to avoid damage. When subjected to violence and non-physiological activity, the ligament is pulled and exceeds its tolerance, and damage occurs.

Cell Characteristics

1) The cells are derived from normal human joint ligament tissue obtained by surgery. 
2) Cell identification: Fibronectin or vimentin was positive for immunofluorescence staining. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: fibroblast-like cells, adherent culture.    

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.