Human Primary Outer Root Sheath Cells

Cell Details

Mesenchymal stem cells are derived from the mesoderm and ectoderm in early development and belong to pluripotent stem cells. MSCs can differentiate into fat, bone, cartilage, muscle, tendon, ligament, nerve under specific induction conditions in vivo or in vitro. , liver, heart muscle, endothelium and other tissue cells. 

Clinical research on mesenchymal stem cells has been carried out in many countries. The United States has approved more than 60 clinical trials. China has also approved a number of clinical trials and entered the stage of research and development of core technology for mesenchymal stem cells. For the treatment of mesenchymal stem cells for the treatment of more than ten refractory diseases, in addition to promoting recovery of hematopoiesis, co-transplantation with hematopoietic stem cells to improve leukemia and refractory anemia, etc., also used for cardiovascular and cerebrovascular diseases, liver Treatment studies of autoimmune diseases such as sclerosis, bone and muscle degenerative diseases, brain and spinal nerve injury, senile dementia, and lupus erythematosus and scleroderma. 

Cell Characteristics

1) The cells are derived from normal human bone marrow blood tissue obtained by surgery. 
2) Cell identification: CD44 immunofluorescence staining was positive, and CD45 immunofluorescence staining was negative. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: fibroblast-like cells, adherent culture.    

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.