Human Primary Nucleus Pulposus Cells

Cell Details

The progenitor cells of B lymphocytes are present in the hematopoietic islands of the fetal liver, and thereafter the site of B lymphocyte production and differentiation is gradually replaced by bone marrow. Mature B cells mainly reside in the lymphoid nodules in the superficial layer of the lymph nodes and in the lymphoid nodules of the red and white pulp of the spleen. B cells can differentiate into plasma cells under antigenic stimulation, and plasma cells can synthesize and secrete antibodies (immunoglobulins), mainly performing humoral immunity of the body. 

The main changes in the various stages of B cell differentiation in the bone marrow are the rearrangement of immunoglobulin genes and the expression of membrane surface markers. During development and differentiation, B cells also undergo selection to remove non-functional gene rearrangement B cells and autoreactive B cells to form a surrounding mature B cell bank. B cell surface has a variety of membrane surface molecules, recognition of antigens, interaction with immune cells and immune molecules, is also an important basis for the isolation and identification of B cells. The surface molecules of B cells mainly include leukocyte differentiation antigen, MHC and various membrane surface receptors. 

Cell Characteristics

1) Cells are derived from peripheral blood. 
2) Cell identification: CD19 immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: suspension culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.