Human Primary Melanoma Cells

Cell Details

T lymphocytes are derived from pluripotent stem cells of the bone marrow, and some of the pluripotent stem cells or pre-T cells in the bone marrow migrate into the thymus and differentiate and mature under the induction of thymus hormone to become immunologically active T cells. T cells are fairly complex heterogeneous, sub-populations that are constantly renewed in the body and can exist at different developmental stages or functions at the same time. T cells can be divided into several sub-populations depending on the function of the immune response: helper T Helper T cells (Th), suppressor T cells (Ts), effector T cells (Te), cytotoxic T cells (Tc), delayed allergic reaction T Delayed type hypersensitivity T cells (Td), amplified T cells (Ta), primitive or natural T cells (Naive T cells), memory T cells (Tm). 

T cells are the main components of lymphocytes, which have various biological functions, such as directly killing target cells, assisting or inhibiting B cells to produce antibodies, responding to specific antigens and mitogens, and producing cytokines, etc. The immune response produced by cells is cellular immunity. There are two main forms of cellular immunity: specific binding to target cells, destruction of target cell membranes, direct killing of target cells, and release of lymphokines, which ultimately expand and enhance immune effects. 

Cell Characteristics

1) Cells are derived from peripheral blood. 
2) Cell identification: CD3 immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: suspension culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.