Human Primary Caudal Tumor Cells

Cell Details

Naive T cells, also known as unsensitized T cells, which mature in the thymus and migrate to peripheral lymphoid tissues (eg, spleen, lymph nodes). It is a type of T cell with surface-differentiating antigens such as CD4, TCR/CD3, CD28, CD62L, CCR7, which is relatively quiescent before antigen stimulation and developed in the thymus. It can be further differentiated into Th1 cells, Th2 cells, Th17 cells, regulatory T cells (Tregs), effector T cells, etc. by antigen stimulation or dendritic cell (DC) presentation. 

Cell Characteristics

1) Cells are derived from peripheral blood. 
2) Cell identification: CD3, CD45RA immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: suspension culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.