Human Primary B Lymphocytes Cells

Cell Details

Natural killer cells (NK) are important immune cells of the body, not only related to anti-tumor, anti-viral infection and immune regulation, but also participate in hypersensitivity and autoimmune diseases in some cases. A LAK-1 molecule stably expressed on the surface of NK and LAK cells, 120 kDa, NK cells were still positive for LAK-1 for 20 days under IL-2 conditions, while HNK-1 (CD57) and CD16 partially disappeared. The killing activity of LAK can be inhibited by anti-LAK-1 McAb. 

Natural killer cell stimulatory factor (NKSF) has a stimulating effect on NK cells. IL-2, IL-12, IFN-α, TNF-α and leukoregulin (LR) have a positive regulatory effect on the activation and differentiation of NK cells. Adding the above cytokines in vitro can significantly enhance the killing activity of NK. Prostaglandins (PG) E1, E2, D2 and adrenocortical hormones have inhibitory effects on NK cell activity. 

Cell Characteristics

1) Cells are derived from peripheral blood. 
2) Cell identification: CD56 and CD16 immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: suspension culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.