Human Primary T Lymphocytes Cells

Cell Details

Endothelial progenitor cells are precursor cells of vascular endothelial cells. Under the stimulation of physiological or pathological factors, studies on the repair of injured blood vessels from bone marrow mobilization to peripheral blood show that endothelial progenitor cells are involved in cardiovascular and cerebrovascular diseases and peripheral vascular diseases. It has an important role in tumor angiogenesis and wound healing, and provides new ideas for the research and treatment of ischemic diseases. 

Endothelial progenitor cells have migratory characteristics, can further proliferate and differentiate into naive endothelial cells, lack the characteristic phenotype of mature endothelial cells, and cannot form a lumen-like structure. Its function is mainly involved in the repair of post-natal ischemic tissue angiogenesis and vascular injury. 

Cell Characteristics

1) Cells are derived from human peripheral blood. 
2) Cell identification: CD31 and CD34 immunofluorescence staining were positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: long fusiform, irregular cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.