Human Primary Hematopoietic Stem Cells

Cell Details

The spleen is an important lymphoid organ with functions such as hematopoiesis, filtration of blood, removal of senescent blood cells, and participation in immune responses . It is also an important place for lymphocyte migration and immune response to occur after antigen stimulation. 

During spleen fibrosis, it is activated by a variety of pathological factors, secreting a variety of extracellular matrices, including collagen, which initiates and promotes the pathological process of fibrosis. Under normal circumstances, spleen stellate cells are in a non-activated quiescent state, spherical. When the spleen is damaged or stimulated by cell growth factors or the like, it changes to an activated state.  

Cell Characteristics

1) Cells are derived from human normal kidney tissue. 
2) Cell identification: Desmin immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: long fusiform, stellate cells, adherent culture. 

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.