Quantity | = | Concentration | x | Volume | x | Molecular Weight |
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1) Click the Molarity Calculation Cart® button.
2) Specify the Concentration and Volume of your desired Molarity.
3) Click Calculate.
4) Your desired Quantity will be calculated and will be put under Quantity: (only for mg)
5) Click Add to Cart and then Proceed to checkout to complete the order. You will receive the quantity as you ordered as solvent-free form.
Alert: If you want to make a solution with a solvent (such DMSO), please add instructions in the notes section (such as 10 nM/1 mL in DMSO) and we will pack and deliver the product as a solution (such as 10 nM solution in 1 mL DMSO). If the product cannot be dissolved in DMSO or you would like to specify your own preferred solvent, please add detailed instructions in the notes section. All solutions must be shipped with an ice bag, costing an additional fee of $20 for S&H.
HUM-i007
Each vial contains >5x105 cells in 1mL volume
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Cell Details
The spleen is an important lymphoid organ with functions such as hematopoiesis, blood filtration, removal of senescent blood cells, and participation in immune responses. It is also an important place for lymphocyte migration and immune response to occur after antigen stimulation.
The spleen is composed of the capsule, trabeculae, white pulp, red pulp, and marginal regions. The connective tissue is mainly composed of fiber-washing white.
Cell Characteristics
1) The cells are derived from normal human spleen tissue.
2) Cell identification: vimentin immunofluorescence staining was positive.
3) The purity of the identified cells is higher than 90%.
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
5) Cell growth mode: long spindle cells, adherent culture.
Transportation and Preservation
Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.
1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.
Product Use
1) This product can only be used for scientific research
2) This product has not passed the audit for living animals and humans directly.
3) This product has not passed the audit for in vivo diagnosis.