Human Primary Lymphatic Endothelial Cells

Cell Details

The spleen is an important lymphoid organ with functions such as hematopoiesis, blood filtration, removal of senescent blood cells, and participation in immune responses. It is also an important place for lymphocyte migration and immune response to occur after antigen stimulation. 
The spleen is composed of the capsule, trabeculae, white pulp, red pulp, and marginal regions. Among them, the reticular structure between the trabeculae forms a fine scaffold of the spleen lymphoid tissue. Smooth muscle cells in the trabeculae can regulate the blood content of the spleen by relaxing or stretching.

Cell Characteristics

1) Cells are derived from human normal spleen tissue. 
2) Cell identification: α-SMA immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: long spindle cells, adherent culture. 

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.