Human Primary Peripheral Blood Mononuclear Cells

Cell Details

The periodontal ligament is composed of dense connective tissue, which contains nerves, blood vessels, lymphocytes and epithelial cells. 

Periodontal ligament fibroblasts are the most important cells in the periodontal ligament. Their function is to participate in the synthesis and absorption of collagen, so that the collagen in the periodontal ligament can be continuously updated; it is also related to matrix formation. 

As the host cell of the periodontal ligament, it participates in the pathological, repair and regeneration process of periodontal tissue. Nowadays, the use of periodontal ligament cells to establish in vitro models has become an important means for people to study periodontal tissue diseases.

Cell Characteristics

1) Cells are derived from normal human dental tissues. 
2) Cell identification: Fibronectin or vimentin was positive for immunofluorescence staining. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: fibroblast-like cells, adherent culture.

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.