Human Primary Splenic Stellate Cells

Cell Details

Periodontal disease is a common and frequently-occurring disease in oral diseases, often leading to destruction or defect of periodontal support tissue. At present, periodontal support tissue reconstruction mainly relies on mechanical, drug or guided tissue regeneration technology. With the rapid development of molecular biology, tissue engineering and stem cell technology, periodontal tissue regeneration engineering technology has become a hot spot in the treatment of periodontal disease. Periodontal ligament stem cell (PDLSC) is one of the key seed cells in periodontal tissue regeneration engineering. Therefore, research on periodontal ligament stem cells has become a hot topic.

Cell Characteristics

1) Cells are derived from normal human dental tissues. 
2) Cell identification: CD146 or STRO-1 immunofluorescence staining was positive. 
3) The purity of the identified cells is higher than 90%. 
4) Does not contain HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
5) Cell growth mode: fibroblast-like cells, adherent culture. 

Transportation and Preservation

Depending on the weather conditions and the distance of transportation, the company negotiates with the customer and chooses one of the following methods.

1) 1mL of frozen cell suspension is placed in a 1.8mL cryotube and placed in a foam incubator filled with dry ice for transport; after receiving the cells, thaw the resuscitated cells as soon as possible for culture. If resuscitation is not possible immediately, Cryopreserved cells can be stored at -80°C for 1 month.
2) T-25 culture flask is filled with complete medium and then transported at room temperature. After receiving the cells, please observe the growth state of the cells under a microscope. If the bottle filling rate exceeds 85%, please carry out the subculture immediately. If there are more cells in suspension, allow the flask to stand overnight in the incubator to help the undead suspension cells to reattach.

Product Use

1) This product can only be used for scientific research 
2) This product has not passed the audit for living animals and humans directly. 
3) This product has not passed the audit for in vivo diagnosis.